Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor‑α‑induced chemokine interleukin‑8 secretion in HT‑29 cell line
By: Yuvaraj, Neelakandan
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Contributor(s): Venkatesan, Arul.
Publisher: Mumbai Wolter Kluwer 2018Edition: Vol. 50(6), November-December.Description: 336-343p.Subject(s): PHARMACOLOGY | Anti‑inflammatory | Antioxidant | Halophilaovalis | HT‑29 | Liquid chromatography‑mass spectrometryOnline resources: Click here
In:
Indian Journal of PharmacologySummary: OBJECTIVES: The present study aims to investigate the anti‑oxidant and anti‑inflammatory properties of seagrass Halophilaovalis sulfated polysaccharide on HT‑29 cell line.SUBJECTS AND METHODS: Monosaccharides composition was identified using liquid chromatography‑mass spectrometry (LC‑MS) and the functional groups were analyzed using Fourier transform‑infrared (FT‑IR) spectroscopy. The antioxidant and anti‑inflammatory potential of crude extract and purified fractions was investigated invitro.RESULTS: FT‑IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC‑MS. Ho‑SP and its fractions showed radical scavenging activity in hydroxyl, 2‑azinobis‑3‑ethylbenzothiazoline‑6‑sulfonic acid, and ferric reducing antioxidant power assay in a dose‑dependent manner. Noticeable anti‑inflammatory activity of purified fraction Ho FrIV (IC50 = 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor‑α (TNF‑α)‑induced interleukin‑8 (IL‑8) secretion (0.27 ng/ml) in HT‑29 cell line.CONCLUSION: Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho‑SP could suppress the TNF‑α‑induced secretion of IL‑8 in HT‑29 and thus could be used as a promising antioxidant and anti‑inflammatory candidate with potential benefits
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School of Pharmacy Archieval Section | Not for loan | 2018287 |
OBJECTIVES: The present study aims to investigate the anti‑oxidant and anti‑inflammatory properties of seagrass Halophilaovalis sulfated polysaccharide on HT‑29 cell line.SUBJECTS AND METHODS: Monosaccharides composition was identified using liquid chromatography‑mass spectrometry (LC‑MS) and the functional groups were analyzed using Fourier transform‑infrared (FT‑IR) spectroscopy. The antioxidant and anti‑inflammatory potential of crude extract and purified fractions was investigated invitro.RESULTS: FT‑IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC‑MS. Ho‑SP and its fractions showed radical scavenging activity in hydroxyl, 2‑azinobis‑3‑ethylbenzothiazoline‑6‑sulfonic acid, and ferric reducing antioxidant power assay in a dose‑dependent manner. Noticeable anti‑inflammatory activity of purified fraction Ho FrIV (IC50 = 43.85 μg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor‑α (TNF‑α)‑induced interleukin‑8 (IL‑8) secretion (0.27 ng/ml) in HT‑29 cell line.CONCLUSION: Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho‑SP could suppress the TNF‑α‑induced secretion of IL‑8 in HT‑29 and thus could be used as a promising antioxidant and anti‑inflammatory candidate with potential benefits
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